Journal: Disease Models & Mechanisms

Article Title: Reduced connexin-43 expression, slow conduction and repolarisation dispersion in a model of hypertrophic cardiomyopathy

doi: 10.1242/dmm.050407

Figure Lengend Snippet: MYH7 403/+ has altered depolarisation and repolarisation in iPSC-CMs. (A) Representative field potentials from MYH7 +/+ (grey, top) and MYH7 403/+ (black, bottom) iPSC-derived cardiomyocytes (iPSC-CMs). (B) Zoomed view highlighting differences in repolarization time (field potential duration; i) and slope of the depolarization complex (red arrows; ii). (C) Violin SuperPlots summarizing depolarization slope for MYH7 +/+ ( n =73 replicates/wells from N =6 differentiations) and MYH7 403/+ ( n =89 replicates/wells from N =6 differentiations) iPSC-CMs showing significantly slower depolarization for MYH7 403/+ iPSC-CMs ( N =6, n =66 wells). (D) Violin SuperPlots showing rate-corrected field potential duration (FPD c ) for MYH7 +/+ ( n =85 replicates/wells from N =6 differentiations) and MYH7 403/+ ( n =111 replicates/wells from N =6 differentiations) iPSC-CMs showing significantly prolonged repolarisation for MYH7 403/+ iPSC-CMs. Error bars show mean of N ±s.e.m. * P <0.05 (Wald test).

Article Snippet: Violin SuperPlots of data were created using Violin SuperPlots in MATLAB ( ); error bars on violin plots represent estimated marginal means of N from the generalised linear model and their standard errors.

Techniques: Derivative Assay

Journal: Disease Models & Mechanisms

Article Title: Reduced connexin-43 expression, slow conduction and repolarisation dispersion in a model of hypertrophic cardiomyopathy

doi: 10.1242/dmm.050407

Figure Lengend Snippet: MYH7 403/+ slows conduction velocity in monolayers of iPSC-CMs. (A) Conduction maps for MYH7 +/+ (i) and MYH7 403/+ (ii) coloured according to activation time measured on a microelectrode array. X- and y -axis labels represent the electrode position in the array. (B) Violin SuperPlots summarising maximum propagation for MYH7 +/+ ( n =64 replicates/wells from N =6 differentiations) and MYH7 403/+ ( n =66 replicates/wells from N =6 differentiations) iPSC-CMs, showing significantly slower conduction in MYH7 403/+ iPSC-CMs. Error bars show mean of N ±s.e.m. * P <0.05 (Wald test).

Article Snippet: Violin SuperPlots of data were created using Violin SuperPlots in MATLAB ( ); error bars on violin plots represent estimated marginal means of N from the generalised linear model and their standard errors.

Techniques: Activation Assay, Microelectrode Array

Journal: Disease Models & Mechanisms

Article Title: Reduced connexin-43 expression, slow conduction and repolarisation dispersion in a model of hypertrophic cardiomyopathy

doi: 10.1242/dmm.050407

Figure Lengend Snippet: Increased spatial dispersion of repolarization in MYH7 403/+ . (Ai) Microelectrode array geometry. (Aii) Typical field potentials measured in an individual array for MYH7 +/+ and MYH7 403/+ iPSC-CMs. (B) Range of field potential durations across the 16 electrodes in ten individual arrays for both MYH7 +/+ (i) and MYH7 403/+ (ii) iPSC-CMs. For each array, individual datapoints represent the field potential duration recorded at an individual electrode in the array. Error bars show mean±s.d. (C) Violin SuperPlots summarizing the standard deviation (SD) of field potential duration (FPD) in each electrode array for MYH7 +/+ ( n =77 wells from N =6 differentiations) and MYH7 403/+ ( n =111 wells from N =6 differentiations) iPSC-CMs, showing significantly more dispersion of repolarization times for MYH7 403/+ iPSC-CMs. Error bars show mean of N ±s.e.m. * P <0.05 (Wald test). (D) Spatial maps of repolarization duration for MYH7 +/+ (i) and MYH7 403/+ (ii) iPSC-CMs.

Article Snippet: Violin SuperPlots of data were created using Violin SuperPlots in MATLAB ( ); error bars on violin plots represent estimated marginal means of N from the generalised linear model and their standard errors.

Techniques: Dispersion, Microelectrode Array, Standard Deviation